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Digital library of scientific journals published by Plovdiv University Paisii Hilendarski.

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Browsing Journals by Author "Aggoun, Moufida"

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    Effect of natural bioflavonoid on in vitro ruminal microbiota activity in sheep rumen liquor
    (Plovdiv University Press “Paisii Hilendarski”, 2017-11-15) Aggoun, Moufida; Boussada, Amina; Arhab, Rabah; Amokrane, Serine; Barkat, Malika
    A pure bioflavonoid (rutin) was extracted from Eucalyptus globulus leaves and identified by Thin Layer Chromatography using purified flavonoids moieties as a control. The purified flavonoid was used in in vitro gas production test to evaluate its effect on rumen fermentation traits of three substrates: vetch-oat hay, alfalfa hay and wheat straw. The concentrations tested were at 0.5 and 1 mg/ml. Globally, the addition of rutin did not affect significantly gas production (P<0.001). Nevertheless, for both levels, rutin caused a slight decrease in methane production (P<0.05). The high reduction was observed for wheat straw (15.53%, 19.6% for 0.5mg/ml and 1mg/ml, respectively). However, in vitro degradability of the three substrates was increased but this increase was not statistically significant (P<0.001). There was not any significant change in PF and microbial biomass production due to the addition of rutin. At same, rutin inclusion did not affect ammonia production of alfalfa hay and vetch-oat hay, but that of wheat straw was significantly decreased (P<0.001). There was not any significant effect on the acetate : propionate ratio. This bioflavonoid has a potential to alter the rumen fermentation pattern, mainly, methane production. Thus, others studies will be conducted to evaluate the dose of administration which will have a maximum reduction in the methane emission and to establish its impact on ruminale microbiota composition especially protozoa and Archaea bacteria.
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    In vitro fermentation of olive oil mill wastewaters using sheep rumen liquor as inoculum: Olive mill wastewaters an alternative for ruminant’s nutrition
    (Plovdiv University Press “Paisii Hilendarski”, 2014-09-27) Aggoun, Moufida; Arhab, Rabah; Leulmi, Nassima; Barkat, Malika
    Olive oil mill wastewaters (OMWW) are the main liquid effluents generated by the olive oil production industry. This liquid, considered pollutant and toxic, is characterised by its high content of organic matter including mainly sugars and fats, and phenols compounds, which can be used in ruminants feeding. The purpose of this study is to valorise this agricultural by-product in ruminant feeding by estimation its in vitro degradability in presence of ovine ruminale microbiota comparatively to vetch-oat hay, using in vitro gas production technique coupled with NH3-N and protozoa measurements. Cumulative gas production was recorded at 3, 6, 9, 24, 48, 72 and 96 hours of incubation. The determination of gazes produced (carbon dioxide and methane) was recorded at 6, 9, 24, 48 and 96 hours. However, Ammonia and protozoa number were recorded after 24 hours of incubation. Fermentation profile was fitted to the exponential model y = a + b (1 – e-kt). The OMWW are characterized by their high sugars content (39.91%) and their low content in ash (1.99%) and crude protein (2.70%). This by-product is also characterized by its high concentration in total phenols (7.2%) and tannins (4.5%). However, they contain a very small amount of condensed tannins (0.89%). Comparatively to vetch-oat hay, OMWW produced low amount of gas (-23.6 units). Furthermore, its in vitro fermentation generates low volume of methane (9.83%, V/V), suggesting that the OMWW nature enhanced the efficiency of ruminale microbiota towards microbial biomass production and inhibition of ruminale methanogenesis pathway. This result is reinforced by the reduction of ammonia production (-0.35 units) and protozoa proliferation (-1 unit) comparatively to vetch-oat hay. The anaerobic biodegradation of OMWW reveal their significant use by the rumen microbiota, allowing us to strongly recommend its use as a supplement in feed ruminant. In addition, it allows considering using this residue as a feed additive in diets of ruminants for the reduction of ruminal methanogenesis.