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Digital library of scientific journals published by Plovdiv University Paisii Hilendarski.
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Browsing Journals by Author "Ali, Md. Akkas"
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Item Assessment of genetic diversity among moderately drought tolerant landraces of rice using RAPD markers(Plovdiv University Press “Paisii Hilendarski”, 2013-09-26) Islam, Md. Shariful; Ali, Md. Akkas; Guswami, Pinak; Ullah, S.M. Sarid; Hossain, Md. Musharaf; Miah, Md. Faruque; Prodhan, Shamsul H.Genetic diversity and relationships among six rice genotypes were investigated using five random amplified polymorphic DNA (RAPD) markers. A total of 69 alleles were amplified, of which 66 were polymorphic. The size of the amplified alleles was between 0.25 and 2.35 kbp. The number of polymorphic alleles detected with each primer ranged from 7 to 24 with an average of 13.2 per primer and the polymorphism information content (PIC) values varied from 0.8672 to 0.9471. Pair-wise similarity estimated the range of 0.308 to 0.718 among all the genotypes and the highest genetic similarity was found between Maloti and BRRI dhan53. Cluster analysis using UPGMA (unweighted pair group method with arithmetic averages) revealed three clusters at genetic similarity of 46%. A moderately drought tolerant landrace, Boalia, formed a single cluster and the remaining genotypes grouped into distinct clusters based on their relatedness. The results showed a high level of genetic diversity among studied genotypes and this information will assist in conservation as well as selection of parents during breeding programs for the development of drought tolerant rice varieties in near future.Item Genetic diversity among salt-tolerant rice (Oryza sativa L.) landraces cultivated in the coastal districts of Bangladesh(Plovdiv University Press “Paisii Hilendarski”, 2013-12-20) Ali, Md. Akkas; Islam, Md. Shariful; Mandal, Santosh Kumar; Nasrin, Zulia; Rahman, Md. Mahbubur; Kuddus, Ruhul H.; Prodhan, Shamsul H.The aim of this study was to determine genetic diversity and relationships among the salt-tolerant rice landraces cultivated in the coastal districts of Bangladesh. DNA extracted from seedlings of nine salt-tolerant local rice landraces and three established salt-tolerant rice varieties was subjected to random amplified polymorphic DNA (RAPD) analysis using a number of random decamer primers. Polymorphic bands generated with five primers were scored and used for determining polymorphic information contents (PIC) and in deriving a dendrogram using the Jaccard similarity coefficient-based unweighted pair-group method with arithmetic means (UPGMA). The five primers generated 84 reproducible bands of the size range 0.24-1.90 kbp and 73% of the bands were polymorphic. The UPGMA dendrogram showed five major clusters at genetic similarity of 0.55. The PIC values ranged from 0.71 to 0.90 with an average of 0.85. The pair-wise similarity index values ranged 28.8-97.1% among the twelve genotypes. The three established rice verities formed a single cluster and the local landraces formed four clusters. Our result revealed high level of genetic diversity among the local rice landraces. The RAPD markers identified could be useful in developing high-yield salt-tolerant rice strains with improved grain quality.