Journal of BioScience and Biotechnology (JBB)
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p-ISSN: 1314-6238 / e-ISSN: 1314-6246
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Browsing Journal of BioScience and Biotechnology (JBB) by Author "Alam, Md. Jahangir"
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Item Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation(Plovdiv University Press “Paisii Hilendarski”, 2015-08-03) Bari, Reduanul; Khan, Md. Anik Ashfaq; Islam, Mohammad Majharul; Sutradhar, Pijush; Alam, Md. Jahangir; Khatun, AsmaTissue culture independent transformation technique in crops is relatively new and of popular interest due to its faster approach and efficiency. The prospect of this technique in the production of transgenic Jute plants with new genetic properties is promising. In the present study, two varieties of each of Corchorus olitorius (var. O-72 and var. OM-1) and C. capsularis (var. CVL-1 and var. BJC-83) were used to observe their transformation ability. Agrobacterium tumefaciens strain LBA4404 was used for transformation, which harbors a binary vector pBI121 containing selectable marker gene nptII, gus (β-glucuronidase) reporter gene and a stress tolerance gene GLY-1. The young shoot tip of the seedlings (20-22 cm) was infected with the bacterial culture. The young leaves were collected after 20-21 days of bacterial culture transformation and thereafter histochemical GUS assay was performed. In the putatively transformed regions of the plants, gus reporter gene was expressed showing blue color in the tissues. Non-transformed plants did not show any color. Among the varieties, the percentage of matured plants showing GUS activity was higher in C. olitorious var. O-72 (80.66%) and OM-1 (73.33%) compared to C. capsularis var. BJC-83 (32.50%) and CVL-1 (40.00%). The result of the study provides an indication that efficiency of transformation by using tissue culture independent direct genetic transformation for the two species of Jute may differ significantly.Item In vitro regeneration and antibacterial activity of Prunus domestica L.(Plovdiv University Press “Paisii Hilendarski”, 2015-02-17) Alam, Md. Jahangir; Barua, RajeshAn effective in vitro culture system for mature nodal segments of European plum (Prunus domestica) was established. Several important aspects of regeneration from nodal explants were studied for adaptation of European plum in Bangladesh. Leaf explants were used for callus induction and ½ MS media supplemented with 1.0 mg/l indole butyric acid (IBA) + 0.5 mg/l naphthalene acetic acid (NAA) induced low efficiency callus. The nodal segments of 1.5 cm were cut from young plants, sterilized and established in vitro. The successful cultures were achieved on MS media supplemented with 2-6 mg/l kinetin (KIN); 2, 3, 5 mg/l benzyleaminopurine (BAP) and 4 mg/l KIN combination with 2 mg/l BAP. The highest number of shoot length (1.20 ±0.15 cm), highest number of shoot (3.00±0.35 cm), and highest number of leaf (14.25±1.45) were observed at KIN 4.0 mg/l. The successful multiple shoots were achieved on MS media with 2-6 mg/l KIN, 4 mg/l KIN combination with 2 mg/l BAP. This indicates that, in general, European plum species is very responsive to in vitro regeneration from nodal segment. The shoots from micro cutting were rooted in MS media supplemented with 1.0 mg/l IBA and with 0.5 mg/l BAP; ½ MS media supplemented with 0.5 mg/l IBA + 0.5mg/l NAA and 1.0 mg/l IBA + 0.5 mg/l BAP. Regenerated plantlets successfully acclimatized to grow vigorously with no apparent phenotypic aberrations. In addition, in sense of commercial value without growth hormone achieved great result. Furthermore, the antibacterial activity of extracts of European plum was evaluated against the human pathogenic bacteria Escherichia coli, Klebsiella pneumonia, Protease mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus by agar disc diffusion method. Most of the human pathogens were resistant against commercial disk. But plum fruit extract showed good inhibition zone result (14.25 mm by crude extract against P. aeruginosa and 12.4 mm by dry extract against S. aureus). The positive results of screening of this plum for antibacterial activity forms primary platform for further phytochemical and pharmacological studies.