Ecologia Balkanica

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p-ISSN: 1314-0213 / e-ISSN: 1313-9940

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    Air quality control in Plovdiv, Bulgaria: the citizens' awareness. Results from a pilot quantitative study
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-29) Mitev, Tihomir; Tchalakov, Ivan; Keskinova, Donka; Parapanov, Petar
    Air pollution is a leading risk factor for individual and public health. It is a major environmental stressor responsible for hundreds of thousands of premature deaths in Europe every year. Monitoring and controlling air quality is about the importance of managing public health, as is ensuring people are aware of the dangers of polluted air and getting them involved in the issue. The article presents a summary of the results and data analysis from a pilot study conducted in May-June 2025 on the awareness, interest, personal engagement, and assessment of citizens in Plovdiv (Bulgaria) – one of the most polluted cities in Europe – regarding the control of air quality in the city.
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    Tautomerism influence on QSAR/QSPR modelling of ecotoxicity and physicochemical properties of chemical compounds
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-29) Kochev, Nikolay; Paskaleva, Vesselina; Jeliazkova, Nina
    The OECD guidelines for QSAR/QSPR (Quantitative Structure Activity/Property Relationship) modelling and ecotoxicity testing play a significant role in ecological studies by providing standardised, scientifically validated methods to assess the environmental impact of chemicals. Laboratory and terrain tests are combined with QSAR/QSPR models, ensuring consistency, reliability and support for regulatory acceptance, enabling screening and prioritisa-tion, global harmonisation and promotion of alternatives to animal testing. Tautomerism is a fundamental structural phenomenon that can significantly influence the predictive reliability of QSAR/QSPR models used for assessing ecotoxicity and physicochemical properties of chemical compounds. We examine how tautomeric variation affects molecular descriptors, data curation, and model performance. We propose practical incorporation of tautomer information into the model development and cheminformatics pipelines to enhance predictive accuracy and regulatory applicability. By generating exhaustive tautomeric ensembles, our approach sup-ports QSAR/QSPR modelling in line with OECD guidelines for regulatory ecotoxicity endpoints, contributing to the development of robust in silico predictions. By addressing the challenges posed by tautomerism, this work advances the use of computational methods in sustainable chemical safety assessment and supports innovation in non-testing approaches.
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    Protected Areas as a Context for Effective Science Communication and the Development of Sustainability Competences
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-29) Epitropova, Ani
    This study analyses how Bulgarian protected areas can serve as platforms for meaningful science communication and as dynamic contexts for developing sustainability competences and supporting environmental conservation efforts. It focuses on how pre-service education students carry out science communication using digital tools and engage with ecological knowledge, how they interpret and communicate information from primary sources related to Bulgarian protected areas, and to what extent this process supports the development of sustainability competences in line with the GreenComp framework. The analysis covers 49 students’ projects focused on protected areas, developed within a structured educational model specifically designed for the study. The analytical approach focuses on three dimensions: (1) scientific accuracy and completeness of the ecological content—including accurate identification of systematic groups, characterized species of flora and fauna, specific ecosystems, and threats; (2) strategies used to communicate scientific information to non-specialist audiences; and (3) the extent to which students demonstrate key sustainability competences, aligned with the GreenComp framework, including: sustainability knowledge, systems thinking, critical thinking, personal and collective responsibility, collaboration, and initiative. The findings reveal competent patterns in information selection, use of visual and emotional tools, and consistent use of scientifically accurate language. Most students present the characteristics of the protected areas in a detailed, engaging, and scientifically sound manner, demonstrating contextual awareness of local environmental challenges and conservation priorities. These patterns support the use of GreenComp not only as a policy instrument but also as a useful analytical tool for measuring ecological awareness and engagement in academic settings. The study contributes to understanding how science communication and competence development intersect in educational settings, positioning future teachers as key mediators between ecology and society.
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    Applying DNA barcoding and ecological DNA approaches for biodiversity monitoring in aquatic ecosystems
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-29) Apostolova, Elena; Iliev, Iliya
    Molecular ecology is reshaping traditional perspectives on natural systems by integrating advanced molecular techniques into ecological research. This integration offers novel insights into long-standing ecological questions and supports the development of more effective conservation strategies and sustainable resource management. The advent of DNA barcoding, which enables precise species identification using short, standardised genomic regions, has significantly improved taxonomic resolution, particularly for taxa with ambiguous or unresolved classifications. In parallel, environmental DNA (eDNA) analysis is revolutioni-sing biodiversity monitoring by facilitating the detection of organisms directly from environmental samples, without the need for physical specimen collection. Together, DNA barcoding and eDNA represent a powerful, non-invasive, and efficient toolkit for studying biodiversity, especially in aquatic ecosystems, where traditional survey methods are often constrained. This review synthesises recent advances in the application of these molecular approaches to aquatic biodiversity monitoring, with a focus on their underlying principles, practical applications, methodological challenges, and prospects.
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    Documentation of the Smooth Snake, Coronella austriaca (Serpentes: Colubridae) from Euboea Island, Greece
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-28) Strachinis, Ilias; Kalogiannis, Stamatios; Katsiyiannis, Philippos; Kouelis, Charis; Stefanopoulos, Platon; Tzoras, Elias
    Euboea, the second-largest island of Greece, supports a diverse range of habitats and remains faunistically understudied, despite its proximity to the mainland and the capital of Greece, Athens. We document the first verified records of the Smooth Snake (Coronella austriaca) on Euboea, based on two individuals observed on Mounts Xirovouni and Dirfi. The species is known in Greece only from mainland areas and has also been historically reported from Samothrace Island based on a single museum specimen. The presence of C. austriaca on Euboea expands the known insular distribution of this predominantly mainland species. Our findings highlight the biogeographical importance of Euboea and underscore the need for further surveys to understand better the distribution, habitat use, and conservation status of C. austriaca, and possibly other overlooked reptile species on the island.
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    Analysis of perennial temperature variations and behavioral ecology of insects for the region of the city of Plovdiv, Bulgaria
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-20) Ivanov, Plamen
    Climate change is significantly affecting both natural ecosystems and human-managed systems on a global scale. The stressors associated with the Anthropocene are diverse and intricate, including invasive species, habitat degradation, pesticide application, and pollution. However, none of these stressors are as pervasive or interconnected with various other factors as climate change. Consequently, understanding the effects of anthropogenic climate change on natural systems is a paramount challenge for environmental sciences in the 21st century. The Intergovernmental Panel on Climate Change (IPCC) defines climate change as “a shift in the climate's state that can be recognized (using statistical methods) by alterations in the average and/or the variability of its attributes, persisting for a long time, generally for decades or longer”. This definition encompasses any changes in climate over time, whether resulting from natural variations or human-induced activities. Climate-related variables, such as temperature, water availability, and carbon dioxide levels, critically affect the characteristics of ecosystems that facilitate food production, including both freshwater and marine environments, agriculture, and forestry. Any fluctuations in these variables present a risk to global food security. This research, therefore, is crucial in understanding and mitigating this risk.
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    Evaluation of the water quality of the rivers used for the irrigation of the Korçë field, Albania
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-27) Maho, Adrian; Ajҫe, Sulltane; Mero, Gjergji; Skenderasi, Besnik
    Water from multiple sources, including rivers, streams, springs, lakes, irrigation reservoirs and groundwater is used to irrigate agricultural crops. Crop productivity depends not only on sufficient amount of water, but also on its good quality. The quality of irrigation water affects not only plant productivity but also product quality and food safety. The study conducted a dynamic assessment of the water quality of two main rivers, the Devoll River and the Dunavec River, which are used for the irrigation of the Korçë field, Albania. The study evidenced the impact that human activity has on water pollution of these various chemicals during the use of water for family purposes, the disposal of waste into rivers, the treatment of agricultural plants with pesticides, and the cutting down of forests. The analyses of physico-chemical and biological indicators were performed according to standard methods in the Agro-food laboratory of the Faculty of Agriculture, Fan Noli University, Korçë. The indicators resulting from the analysis were compared with the standards set by FAO for irrigation water quality and EU Regulation 2020/741. The pollution of the ecosystem of these rivers has led deterioration in their quality indicators. Interventions in wastewater treatment, reforestation, the introduction of biological methods for combating diseases and pests, and the ecological awareness of communities are contributions towards improving the quality of river waters.
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    Study of the agrobiodiversity of the Lotus corniculatus L. grassland under mountain conditions after the application of organic fertilizers
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-23) Churkova, Katerina
    In a research experiment, carried out in 2020-2023 in the Research Institute of Mountain Stockbreeding and Agriculture-Troyan, on the grassland of Lotus corniculatus L., variety "Targovishte 1", the influence of fertilization with the organic fertilizers Blago 5 in doses of 300 and 600 ml/da (varieties 2 and 3) and Fertileader Axis in doses of 500 and 1000 ml/da (varieties 4 and 5) was tested in comparison with an unfertilized control (variety 1). Biodiversity of the untreated crop and agrobiodiversity by botanical groups and weed species composition were studied. The ecological effect of organic fertilization and its impact on the floristic composition of the grassland were determined. The percentage and species contribution of the main botanical groups (grasses, legumes, motley grasses) in the Lotus corniculatus L. grassland changed positively under the influence of fertilization, and the group of motley grasses including plants from different botanical families. There was an increased presence of the shade grass species Lotus corniculatus L. and a reduced number and percentage of representatives of motley grasses in the formed above-ground mass after treatment with the two biofertilizers. Fertileader Axis biofertilizer at a dose of 1000 ml/da proved to be the most effective, stimulating the development of Lotus corniculatus L. in the stand by 82.6% and reducing the number of weedy species (13.6%). The resulting higher relative proportion of Lotus corniculatus L. suggests better quality indicators of grass biomass. The results obtained define the applied biofertilizers as an effective agrotechnical practice, leading to an increase in the presence of economically important grass species and a decrease in the proportion of low-productive variable grasses.
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    Blood biochemistry of White Storks (Ciconia ciconia) across Bulgaria: baseline data for conservation and rehabilitation
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-30) Petrov, Rusko
    We obtained 175 blood samples from wild White Storks in Bulgaria, and determined the values of 18 indicators - alanine transaminase, albumin, alkaline phosphatase, amylase, aspartate transaminase, calcium, chloride, cholesterol, creatine kinase, creatinine, blood glucose, lactate dehydrogenase, magnesium, phosphorus, total bilirubin, total protein, triglycerides and uric acid. In this research, we compared those results with regards to the location from which the birds came from - we conditionally separated Bulgaria into four geographical regions, and to their age. We found statistically significant differences in two parameters - albumin, between northwestern and northeastern populations, and creatinine, between northeastern and southeastern populations. There was also a statistically significant difference in the albumin values between juvenile and adult storks, which we found to be decreasing with age.
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    Assessment of the quality of groundwater for irrigation in the municipality of Suharekë, Republic of Kosovo
    (Plovdiv University Press "Paisii Hilendarski", 2025-04-28) Çadraku, Hazir; Shala, Agron; Xhafa, Sokol
    This study evaluates groundwater quality for irrigation in Suharekë, Kosovo. Sixteen well samples were analyzed for cations (Na+, K+, Ca2+, Mg2+), anions (Cl-, NO3-, SO42-, HCO3-, CO32-), and irrigation quality indices (SAR, Na%, KR, MAR, PI, PS, RSC). Results indicate groundwater suitability for irrigation, with SAR (excellent), SP (excellent), KR (suitable), MAR (62.5% suitable, 37.5% unsuitable), PI (suitable), PS (excellent-good), and RSC supporting usability.
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    Soil carbon and nitrogen stocks under anthropogenic influence: A case study in European beech forests of Central Balkan Mountains (Stara Planina), Bulgaria
    (Plovdiv University Press "Paisii Hilendarski", 2025-06-03) Kirova, Lora; Stoeva, Lora; Stoyanov, Todor
    Understanding how forest management practices influence soil carbon and nitrogen dynamics is crucial for sustainable ecosystem functioning and climate mitigation. This study investigates the effects of thinning on soil organic carbon (SOC) and total nitrogen (TN) stocks in temperate European beech (Fagus sylvatica L.) forests in the Central Balkan Mountains of Bulgaria. Fieldwork was conducted in six forest districts using a replicated plot design with three thinning intensities: unmanaged (0%), moderate (10–15%), and higher (20–25%). Soil samples were collected from three depth intervals (0–10, 10–20, and 20–30 cm) and analyzed for SOC, TN, and related properties following standardized laboratory protocols. Results showed that SOC and TN concentrations decreased consistently with depth across all treatments. While higher thinning intensity was associated with modest reductions in subsoil SOC and TN, the overall stocks to 30 cm depth did not differ significantly between managed and control plots. The data suggest that brown forest soils (Cambisols) exhibit strong resilience to moderate thinning, likely due to conservative harvesting practices, stable canopy structure, and the retention of organic inputs. These findings highlight that moderate-intensity thinning, as applied under Bulgarian forestry regulations, can be compatible with the preservation of soil carbon and nitrogen pools. The study emphasizes the importance of site-specific management and long-term monitoring to ensure soil sustainability under changing climatic and land-use conditions.
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    Study of basic indicative parameters for soil health in beech forests of Petrohan area, Western Bulgaria
    (Plovdiv University Press "Paisii Hilendarski", 2025-05-22) Petrova, Kameliya
    Soils in forest areas are one of the most vulnerable terrestrial ecosystems, which are subject to sensitive changes because of anthropogenic impact and global climate changes. Studying the main soil indicators on which soil health depends is essential to setting realistic goals for its maintenance and/or improvement. The study focuses on assessment of basic indicative soil parameters, which are directly related to the health status of the soil. A total of 10 soil profiles of Dystric Cambisols from Western Bulgaria were studied. The soil profiles were assessed as shallow. The average depth of A horizon was 11 cm. Very low pH values were established in A (3.7 to 5.3) and Bw horizons (3.8 to 4.7) of the soils which indicate ongoing acidification processes in soil depth. The studied soils were assessed as porous with ideal bulk density values. They were moderately (144 t/ha) to very well stocked (665 t/ha) with humus and total nitrogen, but poor on available potassium and phosphorous. All the soils were assessed as unsaturated with bases. Their base saturation (BS) values ranging between 5% and 34%. The obtained results showed that low soil depth, acidic soil reaction, low BS, and cation exchange capacity (CEC) are the main prerequisites for the deterioration of soil health. The studied soils are sensitive to acidification processes, which can lead to poor soil health status and, therefore, to soil degradation. It is highly recommended to continue the monitoring on the health status of these soils, because forest soils are becoming more and more susceptible to degradation processes.
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    Analysis of medicinal plants from the regions of the Strandzha mountain and southern Black Sea Coast, Bulgaria
    (Plovdiv University Press "Paisii Hilendarski", 2025-05-07) Todorov, Krasimir; Stoyanov, Plamen; Mladenov, Rumen; Gyuzeleva, Donika; Radoukova, Tzenka; Selyami, Dzhansu; Zhelyazkova, SImona; Mladenova, Tsvetelina
    This article presents summarized information about the medicinal plants distributed on the territory of Strandzha mountain and the southern Black Sea coast, Bulgaria. According to literature data, 252 medicinal taxa are found on the territory of the two floristic regions, 218 of which are included in the Medicinal Plants Act. All species included in the list are characterized by: biological type, life form, and floral element. 59 plants have conservation status, representing 23.4% of the established species composition. According to the Red List of Higher Plants in Bulgaria and the Red Book of the Republic of Bulgaria, the following species are critically endangered (CR): Arbutus unedo L. and Sideritis syriaca L. Four Balkan endemics have been identified - Achillea clypeolata Sm., Opopanax chironius (L.) W. D. J. Koch subsp. bulgaricum (Velen.) Andreev, Sempervivum leucanthum Pančič, and Thymus longedentatus (Deg. & Urum.) Ronn.
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    Ecological effects of changes in the ignition angle of a gasoline engine on exhaust harmful gases emissions
    (Plovdiv University Press "Paisii Hilendarski", 2025-05-28) Kanevski, Hristo; Lyubomirov, Slavi; Asenov, Stanislav; Shehova, Daniela
    The present study reports the results from a real experiments of the impact of the ignition angle on the harmful exhaust gases emitted by a gasoline engine. The experiments were carried out with a BMW 318 gasoline engine equipped with a MegaSquirt 3 electronic control unit and TunerStudio MS software, as well as a Kane AUTOplus gas analyzer. The main emissions considered in the study include some greenhouse gases such as carbon monoxide (CO), carbon dioxide (CO2) and unburned hydrocarbons (HC). The emissions of harmful gases were measured at 7 different engine speeds and 9 different ignition angles. The obtained data were used to compile regression equations that describe the relationship between the ignition angle, engine speed and the harmful emissions levels. The obtained results and derived equations offer specific recommendations for optimization of the ignition angle to minimize harmful emissions and improve the environmental performance of gasoline engines.
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    Paths to Sustainable Development: Ecological (R)evolution
    (Plovdiv University Press "Paisii Hilendarski", 2025-05-23) Dakova, Megi; Nikolov, Bogdan; Getzova, Viktoriya
    There is increasing talk about the need for mechanisms to reduce the pressure on our planet and the benefits of moving towards a green economy. In this regard, attention is focused on various packages of economic indicators, including several stages - from the creation of the product and its consumption to the need for waste management at the end of its life cycle. The circular economy is a model aimed at extending the life cycle of products. The aim of this study is to synthesize the main strategies and principles for sustainable waste management, the pros and cons of the circular economy, as well as to outline some potential paths for the transition to a circular economy. The relevance of the study is determined by modern trends in harmonizing policies and national measures for the management of packaging and packaging waste. Improving the quality of the environment by preventing and reducing the impact of packaging and packaging waste, preventing the production of packaging waste and promoting the reuse, recycling and other forms of recovery of packaging waste instead of its final disposal, thus contributing to the transition to a circular economy.
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    Laccase-Based Biosensors: Advancements and Applications in Environmental, Biochemical and Biomedical Sensing
    (Plovdiv University Press "Paisii Hilendarski", 2025-04-23) Peshkov, Angel; Iliev, Ilia; Dimcheva, Nina
    Laccase-based biosensors represent a promising and innovative area of biotechnology that harnesses the natural enzymatic properties of laccase, a copper-containing oxidative enzyme, for detecting various aromatic compounds. These biosensors have garnered attention due to their high specificity, sensitivity, and eco-friendly nature, making them suitable for multiple applications, including environmental monitoring, biomedical diagnostics, and industrial process control. This overview provides insights into the fundamental principles, advantages, and key applications of laccase-based biosensors, along with emerging trends and challenges in this rapidly advancing field.
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    Some aspects of the impact of photovoltaic plants on the environment
    (Plovdiv University Press "Paisii Hilendarski", 2025-05-23) Petrova, Slaveya; Nikolov, Bogdan; Popov, Rumen; Lyubomirov, Slavi; Asenov, Stanislav; Kanevski, Hristo; Dakova, Megi; Yordanov, Emil; Kazandzhieva, Svetlozara; Klimentova-Nikolova, Yanina
    Renewable energy sources (RES) play a key role in the global effort to tackle climate change and ensure a sustainable energy future. They are energy sources that are naturally renewable and have a minimal or zero carbon footprint. The main types of renewable energy sources include wind, solar (thermal and photovoltaic), aerothermal, geothermal, hydrothermal, ambient energy, tidal, wave and other ocean energy, hydroelectric power, biomass, landfill gas, sewage treatment plant gas and biogases (EC Directive 2018/2001). Large-scale photovoltaic power plants (PPPs) are being developed at a rapid pace and are set to use thousands or millions of acres of land worldwide. While the energy, economic and environmental impacts of PPPs are generally considered positive, large-scale deployment has negative impacts on non-urban areas. Specific impacts on soils and rural areas (e.g. permanent or temporary soil sealing conditions, complete or partial soil shading, land degradation, habitat fragmentation and loss of traditional agricultural practices) have been identified and require further investigation. The life cycle analysis of PPPs showed that they cannot be accepted as green technologies with zero emissions due to the potential negative effects on the environment. However, they are one of the most promising renewable energy sources, which emphasizes the need to search for sustainable models for their production, construction and operation.
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    Photovoltaic power plants on agricultural land – are they really green?
    (Plovdiv University Press "Paisii Hilendarski", 2025-01-22) Klimentova-Nikolova, Yanina; Petrova, Slaveya; Nikolov, Bogdan; Popov, Rumen; Lyubomirov, Slavi; Asenov, Stanislav; Kanevski, Hristo; Dakova, Megi; Angelov, Nikola; Kazandzhieva, Svetlozara; Yordanov, Emil
    Photovoltaic systems are a rapidly developing technology that plays a crucial role in the transition to clean energy and the reduction of greenhouse gas emissions. However, it is important to consider their potential impacts on the environment and biodiversity, especially when installing them on agricultural land. The aim of this study is to analyze and assess the impact of photovoltaic power plants (PPPs) on soil properties, microclimate and biodiversity. Renewable energy infrastructure can impact biodiversity in different ways during its construction, operation and maintenance, as well as during decommissioning or recommissioning. Potential impacts of PPPs on biodiversity include direct morbidity and mortality of wildlife species, habitat loss and degradation, habitat fragmen-tation and barrier effects, habitat alteration or creation, behavioral changes, physiological changes and displacement, infrared wave impacts, impacts on ecosystem services, indirect impacts, and cumulative impacts at the population level. Constructions with photovoltaic panels can affect soil and microclimate conditions by trapping precipitation and atmospheric deposition, changing surface albedo, increasing ground shading, and influencing wind speed. Significant changes in these parameters have been found in all studied locations, which can lead to changes in species composition, richness and diversity. The results obtained for the main negative impacts of PPPs on agricultural lands on the environment can be used to solve a wide range of management tasks such as control over investment proposals in the field of photovoltaics, etc.
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    Influence of Camelina crops on soil CO2 emissions
    (Plovdiv University Press "Paisii Hilendarski", 2025-02-03) Petrova, Slaveya; Stanchev, Georgi; Marcheva, Marina; Popov, Vladislav
    The Green Deal poses different challenges for EU agriculture, and this production will cost more and will be reflected on the global market once agriculture is included in the emissions trading system. Sustainable land management will be crucial to achieving the EU's climate neutrality target by 2050, as it will increase the amount of carbon captured and stored in plants and soils. Agriculture’s role in carbon sequestration is most closely linked to soil as a carbon sink. Soils have the potential to act as significant carbon sinks, storing carbon that would otherwise be released into the atmosphere as CO2. Through practices such as cover crops, reduced tillage, and organic farming, farmers can improve soil carbon sequestration, contributing to climate mitigation efforts. The aim of the present study was to track the dynamics of soil CO2 emissions during the vegetation of Camelina in different intercropping systems and, on this basis, to evaluate the possibilities for sustainable management of carbon in the soil. After the three-year studies, we can recommend the use of Camelina as monocultures and especially in mixed crops with legumes as a step towards sustainable management of CO2 emissions and towards the so-called carbon agriculture. This approach has the additional advantage of biologically nourishing the soil with nitrogen, as well as creating more favorable conditions for the development of the soil microbiome.
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    First record of chestnut blight on Quercus petraea (Fagaceae) in Bulgaria
    (Plovdiv University Press "Paisii Hilendarski", 2025-03-18) Filipova, Eva; Manova, Vasilissa; Bonchev, Georgi; Boycheva, Irina; Georgieva, Margarita
    Chestnut blight is one of the most destructive diseases on species of Castanea genus (Fagaceae). The causal agent of the disease is the ascomycete fungus Cryphonectria parasitica (Valsaceae). In the period 2018-2020, chestnut blight was found on both European (sweet) chestnut (Castanea sativa) and sessile oak (Quercus petraea) trees in mixed chestnut-oak stands planted in the region of State Forest Enterprise (SFE) Simitli (Pirin Mt.) and SFE Petrich (Belasitsa Mt.). The present study provides new data on the first detection of damage caused by Cryphonectria parasitica on Quercus petraea plantations in southwestern Bulgaria. The frequency and impact of the disease, the variety of vegetative compatibility types of the pathogen, as well as the molecular identification and mating type of the pathogen from oak samples were characterized.