Micropropagation of Helichrysum italicum (ROTH) G. Don – a medicinal plant with ornamental value
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Date
2019-02-14
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Plovdiv University Press “Paisii Hilendarski”
Abstract
Helichrysum italicum (Roth) G. Don is a Mediterranean vegetal species from the Asteraceae family. The ornamental value of the flowers and the properties of its essential oils contribute to its popularity. The purpose of present study was to develop methods for in vitro micropropagation of this medicinal and ornamental plant. In order to improve shoot growth, development and proliferation nutrient media based on both MS (Murashige and Skoog, 1962) or DKW (Driver and Kuniyuki, 1984) formulations were used. They were enriched with different cytokinins - BAP, Kinetin, or Zeatin (5µM). The best multiplication rate was achieved on DKW medium, supplemented with 5µM Kinetin. High percentage of rooting was achieved on the control treatment and the nutrient media, supplemented with IBA.
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Keywords
tissue culture, ornamental and medicinal plants, multiplication rate