Application of PCR-RFLP method to determine polymorphism in BMP-15 and GDF9 fecundity genes in Northeast Bulgarian Merino sheep breed
| dc.contributor.author | Bozhilova-Sakova, Milena | |
| dc.contributor.author | Dimitrova, Ivona | |
| dc.date.accessioned | 2024-11-19T05:48:22Z | |
| dc.date.available | 2024-11-19T05:48:22Z | |
| dc.date.issued | 2021-12-06 | |
| dc.description.abstract | The purpose of this study was to determine the presence or absence of mutations in BMP-15 and GDF9 fecundity genes. In this experiment 60 ewes from Northeast Bulgarian Merino sheep breed were tested for polymorphisms in BMP-15 gene and SNP G1 of the GDF9 gene. This is the first investigation of BMP-15 gene by PCR-RFLP method in Bulgarian sheep breed. After DNA extraction and PCR amplification were received the expected PCR products. The digestion with restriction enzyme HinfI for BMP-15 gene and HhaI for GDF0 revealed mutations in both genes. In BMP-15 were found alleles + and G with frequencies 0.94 and 0.06, respectively and genotypes ++ and G+ with frequencies 0.80 and 0.12, respectively. In GDF9 were identified alleles G and A with frequencies 0.94 and 0.06 and genotypes GG and AG with frequencies 0.88 and 0.12. | |
| dc.identifier.issn | 1314-6246 | |
| dc.identifier.uri | https://doi.uni-plovdiv.bg/handle/store/324 | |
| dc.language.iso | en | |
| dc.publisher | Plovdiv University Press “Paisii Hilendarski” | |
| dc.subject | Northeast Bulgarian Merino sheep breed | |
| dc.subject | BMP-15 | |
| dc.subject | GDF9 | |
| dc.subject | PCR-RFLP analysis | |
| dc.subject | polymorphism | |
| dc.title | Application of PCR-RFLP method to determine polymorphism in BMP-15 and GDF9 fecundity genes in Northeast Bulgarian Merino sheep breed | |
| dc.type | Article |