Staphylococcus aureus enterotoxin A gene induce the changes in the expression of apoptosis-related genes in AGS cell line

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dc.contributor.authorAfzali, Sara
dc.contributor.authorDoosti, Abbas
dc.contributor.authorHeidari, Mansour
dc.contributor.authorBabaei, Nahid
dc.contributor.authorKeshavarz, Parvaneh
dc.date.accessioned2024-11-19T05:47:53Z
dc.date.available2024-11-19T05:47:53Z
dc.date.issued2021-12-06
dc.description.abstractGastric cancer is one of the most common malignancies worldwide and it is the fourth leading cause of cancer-related death. Staphylococcus aureus enterotoxin A (SEA) is one of the toxins affecting the expression of apoptotic-related genes. The aim of this research is to investigate the effects of S. aureus enterotoxin A on the expression of BAK, FAS, BAX, TNF-α, BCL-2 and Survivin in AGS cell lines. In this study, AGS cells were transfected with the pcDNA3.1(+)-enterotoxin A or empty pcDNA3.1(+) plasmids using Lipofectamine 2000. The cells were grown in Dulbecco's modified Eagle's medium (DMEM) with 10% FBS. Then, the mRNA expression level of BAK, FAS, BAX, TNF-α, BCL-2 and Survivin was measured by qRT-PCR method. Our findings indicated that Staphylococcus aureus enterotoxin A markedly altered the expression level of the apoptotic-related gene of the AGS cell line. The mRNA expression of BAK, FAS, BAX and TNF-α genes increased statistically significant in enterotoxin A treated AGS cells compared with the control group. On the contrary, BCL2 and Survivin mRNA expression showed a significant decrease compared with AGS cells were transformed by empty pcDNA3.1(+) plasmid. We suggested that SEA could represent an agent that can significantly changes apoptosis-related genes expression in AGS cell line, leading to reduction of tumor cell proliferation.
dc.identifier.issn1314-6246
dc.identifier.urihttps://doi.uni-plovdiv.bg/handle/store/322
dc.language.isoen
dc.publisherPlovdiv University Press “Paisii Hilendarski”
dc.subjectStaphylococcus aureus
dc.subjectenterotoxin A
dc.subjectAGS
dc.subjectgastric cancer
dc.subjectapoptosis-related gene
dc.titleStaphylococcus aureus enterotoxin A gene induce the changes in the expression of apoptosis-related genes in AGS cell line
dc.typeArticle
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