Influence of composition on the biological activity of pectic polysaccharides from leek
| dc.contributor.author | Ognyanov, Manol | |
| dc.contributor.author | Nikolova, Mariana | |
| dc.contributor.author | Yanakieva, Irina | |
| dc.contributor.author | Kussovski, Veselin | |
| dc.contributor.author | Kratchanova, Maria | |
| dc.date.accessioned | 2024-05-25T19:57:47Z | |
| dc.date.available | 2024-05-25T19:57:47Z | |
| dc.date.issued | 2013-03-08 | |
| dc.description.abstract | Water-soluble and acid-soluble pectic polysaccharides with significant immunostimulating activity were isolated from alcohol-insoluble solids (AIS) of leek. Pectic polysaccharides characterized with high anhydrouronic acid content and common pectic sugars – L-arabinose, D-galactose, D-glucose, and L-rhamnose. It was accomplished that D-galactose content was the highest, which allowed the assumption that pectin sugar side chains were from galactan type. Enzyme modification of acid-extracted pectin was realized. Different pectin-degrading enzymes were used. The first modified pectic fraction, obtained after endopolygalacturonase action and alcohol precipitation contained rhamnogalacturonan I and had high D-galactose content. The second fraction obtained from the first, after β-galactosidase treatment characterized with low D-galactose content. Probably this was result from β-galactosidase hydrolysis of pectin side chains. It was proved by the action of β-galactosidase that galactan side chains were mainly composed of β-(1→4)-linked D-galactose. It was accomplished that rhamnogalacturonan I had higher immunostimulating activity than the initial pectic polysaccharide, which confirmed the statement that pectin hairy regions were responsible for the biological activity. The second fraction had lower D-galactose content and lower activity than the first. Probably galactan side chains were responsible for the immunostimulating activity. | |
| dc.identifier.issn | 1314-6246 | |
| dc.identifier.uri | https://doi.uni-plovdiv.bg/handle/"store"/39 | |
| dc.language.iso | en | |
| dc.publisher | Plovdiv University Press “Paisii Hilendarski” | |
| dc.subject | leek | |
| dc.subject | pectic polysaccharides | |
| dc.subject | pectic enzymes | |
| dc.subject | immunostimulating activity | |
| dc.title | Influence of composition on the biological activity of pectic polysaccharides from leek | |
| dc.type | Article |