Cloning and expression of gene encoding P23 protein from Cryptosporidium parvum

Simple item page
dc.contributor.authorLan, Dinh Thi Bich
dc.contributor.authorLan, Tran Thuy
dc.contributor.authorViet, Le Quoc
dc.contributor.authorQuyet, Phan Van
dc.contributor.authorQuang, Hoang Tan
dc.contributor.authorLoc, Nguyen Hoang
dc.contributor.authorLong, Phung Thang
dc.date.accessioned2024-05-27T20:09:16Z
dc.date.available2024-05-27T20:09:16Z
dc.date.issued2014-09-27
dc.description.abstractWe cloned the cp23 gene coding P23 (glyco)protein from Cryptosporidium parvum isolated from Thua Thien Hue province, Vietnam. The coding region of cp23 gene from C. parvum is 99% similar with cp23 gene deposited in NCBI (accession number: U34390). SDS-PAGE and Western blot analysis showed that the cp23 gene in E. coli BL21 StarTM (DE3) produced polypeptides with molecular weights of approximately 37, 40 and 49 kDa. These molecules may be non-glycosylated or glycosylated P23 fusion polypeptides. Recombinant P23 protein purified by GST (glutathione S-transferase) affinity chromatography can be used as an antigen for C. parvum antibody production as well as to develop diagnostic kit for C. parvum.
dc.identifier.issn1314-6246
dc.identifier.urihttps://doi.uni-plovdiv.bg/handle/store/99
dc.language.isoen
dc.publisherPlovdiv University Press “Paisii Hilendarski”
dc.subjectcp23 gene
dc.subjectCryptosporidium parvum
dc.subjectP23 protein
dc.titleCloning and expression of gene encoding P23 protein from Cryptosporidium parvum
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
jbb20143189.pdf
Size:
1.01 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
73 B
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
Journal of BioScience and Biotechnology (JBB)