Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method

dc.contributor.authorVrancheva, Radka
dc.contributor.authorIvanov, Ivan
dc.contributor.authorMarchev, Andrey
dc.contributor.authorPavlov, Atanas
dc.date.accessioned2024-05-25T19:40:00Z
dc.date.available2024-05-25T19:40:00Z
dc.date.issued2013-02-08
dc.description.abstractA rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – ammonium hydroxide (80:80:40:0.05, v/v/v/v). The results obtained by this method (CV% 3.4) corresponded well with those obtained by using a HPLC method. The reliability of the proposed method was proved through reproducibility test with alkaloid extracts from Fumaria spp.
dc.identifier.issn1314-6246
dc.identifier.urihttps://doi.uni-plovdiv.bg/handle/"store"/36
dc.language.isoen
dc.publisherPlovdiv University Press “Paisii Hilendarski”
dc.subjectTLC
dc.subjectprotopine
dc.subjectHPLC
dc.subjectFumaria officinalis L.
dc.subjectFumaria rostellata Knaf.
dc.titleQualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method
dc.typeArticle
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