Fungal endophytes of high altitude ethnomedicinal plants as a bioresource of industrially pertinent enzymes
| dc.contributor.author | Kapoor, Neha | |
| dc.contributor.author | Mushtaque, Abu | |
| dc.contributor.author | Gambhir, Lokesh | |
| dc.date.accessioned | 2024-11-19T05:52:37Z | |
| dc.date.available | 2024-11-19T05:52:37Z | |
| dc.date.issued | 2021-05-17 | |
| dc.description.abstract | Endophytic fungi have been in the spotlight as a reservoir of novel agents with diverse bioactivities. Similarity in chemical diversity with the host plant makes them an amenable target for industrial interventions. A wide range of compounds as secondary metabolites and enzymes are manufactured inside the endophytic fungal factory. However, utilization of endophytic fungi as industrially imperative enzyme producers has been a scarce event. The present study was conducted to bio-prospect the fungal endophytes present in the high altitude medicinal plants of Uttarakhand, as industrially imperative enzyme producers. A total of 58 different endophytic isolates were obtained from Pinus sabiniana, Cinnamomum tamala, Cinnamomum verum, Ocimum tenuiflorum and Rhododendron arboreum. Endophytic fungal colonization was highest, 31%, in Pinus sabiniana. The pure isolates were further explored for the production of amylases, cellulases, proteases and L-asparaginase. Out of 58 isolates, 40 isolates exhibited potent enzyme productivity. #7PSSTB isolate was considered as a superlative contender on account of its relatively higher production of all the three enzymes viz. amylases, cellulases, proteases. Partial purification of #7PSSTB extract showed compelling enzymatic activity corroborating the existence of exogenous enzyme in the extract. Interestingly, #9 RASTB, #11 RASTB and #17 RASTB exhibited the production of therapeutically imperative L-asparaginase enzyme. The present study puts the spotlight on endophytic diversity in the high altitude medicinal plants as a source of enzymes of industrial interest. Production of L-asparaginase paves the way of pharmaceutical intervention to explore anti-oncogenic effects in the endophytic fungal repository of high altitude regions. | |
| dc.identifier.issn | 1314-6246 | |
| dc.identifier.uri | https://doi.uni-plovdiv.bg/handle/store/338 | |
| dc.language.iso | en | |
| dc.publisher | Plovdiv University Press “Paisii Hilendarski” | |
| dc.subject | L-asparaginase | |
| dc.subject | amylase | |
| dc.subject | proteases | |
| dc.subject | cellulases | |
| dc.subject | endophytic fungi | |
| dc.title | Fungal endophytes of high altitude ethnomedicinal plants as a bioresource of industrially pertinent enzymes | |
| dc.type | Article |