Thiamethoxam causes histochemical changes in the liver of Aristichthys nobilis Rich., 1845
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Date
2015-11-11
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Publisher
Plovdiv University Press “Paisii Hilendarski”
Abstract
In the present study, we aimed to investigate the effects of the neonicotinoid insecticide thiamethoxam on the hepatic glycogen in bighead carp (Aristichthys. nobilis Rich.). Fish were exposed to 6.6 mg/L, 10 mg/L and 20 mg/L of the insecticide under laboratory conditions for 96 hours. The PAS-reaction was applied to liver cryostat sections in order to indicate the amount of glycogen. The results showed that the hepatic glycogen amount increased with increasing the insecticide concentrations. On the other hand, we observed glycogen conglomerates in certain hepatocytes. Hence, our results demonstrated an enhanced process of glyconeogenesis in the fish liver under the influence of thiamethoxam.
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Keywords
histochemistry, PAS-reaction, thiamethoxam, liver, glycogen, bighead carp, Aristichthys nobilis