Influence of in vitro cultivation factors on micropropagation and alkaloid determination of Convolvulus persicus L.
| dc.contributor.author | Doycheva, Iva | |
| dc.contributor.author | Doncheva, Tsvetelina | |
| dc.contributor.author | Philipov, Stefan | |
| dc.date.accessioned | 2026-01-30T10:39:17Z | |
| dc.date.available | 2026-01-30T10:39:17Z | |
| dc.date.issued | 2026-01-25 | |
| dc.description.abstract | Convolvulus persicus L. is a critically endangered species native to Bulgaria. Studying the conditions for its in vitro cultivation is important for its ex-situ conservation. A number of factors influence the growth, development, and multiplication of in vitro plants. In this study, the effects of several medium components - including the type of gelling agent, the quantity of macrosalts, activated charcoal supplementation, and plant growth regulators - were investigated to determine the most suitable conditions for the in vitro cultivation of the species. The effects of explant type and culture vessels were also examined. The type of gelling agent (Gelrite) and the explant significantly affected culture growth. Culture vessels and plant growth regulators had a smaller influence, while the effect of activated charcoal depended on its combination with the other factors. The quantity of macrosalts did not influence plant growth in vitro. A phytochemical analysis of the alkaloid content of Convolvulus persicus was conducted for the first time. The phytochemical analysis revealed that three alkaloids - nicotine, tropinone, and Nmethylpyrrolidinyl-cuscohygrine - were biosynthesized by C. persicus at two developmental stages. Nicotine was identified as the major alkaloid in the species. | |
| dc.identifier.issn | 1313-9940 | |
| dc.identifier.uri | https://doi.uni-plovdiv.bg/handle/store/976 | |
| dc.language.iso | en | |
| dc.publisher | Plovdiv University Press "Paisii Hilendarski" | |
| dc.subject | gelling agent | |
| dc.subject | macrosalts | |
| dc.subject | activated charcoal | |
| dc.subject | culture vessels | |
| dc.subject | explant type | |
| dc.subject | alkaloids | |
| dc.title | Influence of in vitro cultivation factors on micropropagation and alkaloid determination of Convolvulus persicus L. | |
| dc.type | Article |