Biosynthesis of highly unsaturated fatty acids by hydrocarbon degrading microorganisms
| dc.contributor.author | Ghasemi, Mehdi | |
| dc.contributor.author | Atakishiyeva, Yemen | |
| dc.date.accessioned | 2024-05-28T18:08:36Z | |
| dc.date.available | 2024-05-28T18:08:36Z | |
| dc.date.issued | 2015-03-08 | |
| dc.description.abstract | Disruption of polyunsaturated fatty acids (PUFA) metabolism leads to many diseases. In this study, producers of γ-linolenic acid (GLA), arachidonic acid (ARA) and eicosapentaenoic acid (EPA) were selected: Cephalosporium humicola IE (on glucose, dry biomass – 14 g/l, total lipids – 18-20%, GLA in lipids – 12.0%), Mucor globosus 11 (respectively – 15 g/l, 18% and 5%) and Pythium irregulare LX (on glucose, dry biomass – 14.5 g/l, total lipids – 18-20%, 9.2 and 7.8% of ARA and EPA, respectively). On crude oil as the only source of carbon, the amount of biomass of the specified fungi decreases by 3-4 times, whereas the quantity of lipids and highly unsaturated fatty acids increases in four and 1.2 - 3.4 times, respectively. The maximum γ-linolenic acid in M. globosus and C. humicola was detected at neutral рН. Optimum volume of inoculate was 2.0-4.0%, nitrogen source NH4NO3, a carbon-nitrogen ratio 34:1. For biosynthesis of ARA and EPA by P. irregulare, the optimum nitrogen source was NH4Cl, рН 7.0- 8.0 and С/N - 50:1 at 28°C. The process of adaptation to stressful situation under crude oil motivated the increase of the rate of membrane phospholipids with high quantity of unsaturated fatty acids. | |
| dc.identifier.issn | 1314-6246 | |
| dc.identifier.uri | https://doi.uni-plovdiv.bg/handle/store/121 | |
| dc.language.iso | en | |
| dc.publisher | Plovdiv University Press “Paisii Hilendarski” | |
| dc.subject | Cephalosporium humicola | |
| dc.subject | Mucor globosus | |
| dc.subject | Pythium irregulare | |
| dc.subject | single cell oils | |
| dc.subject | polyunsaturated fatty acids | |
| dc.subject | omega | |
| dc.subject | optimization | |
| dc.title | Biosynthesis of highly unsaturated fatty acids by hydrocarbon degrading microorganisms | |
| dc.type | Article |