Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis
| dc.contributor.author | Kostova, Zhivka | |
| dc.contributor.author | Batsalova, Tsvetelina | |
| dc.contributor.author | Moten, Dzhemal | |
| dc.contributor.author | Teneva, Ivanka | |
| dc.contributor.author | Dzhambazov, Balik | |
| dc.date.accessioned | 2024-05-29T18:08:42Z | |
| dc.date.available | 2024-05-29T18:08:42Z | |
| dc.date.issued | 2015-09-13 | |
| dc.description.abstract | Characterization of the pollen allergens and identification of the main T-cell and B-cell epitopes is of crucial importance, since the clarification of the cellular and molecular mechanisms will allow long-term control of this type allergic diseases and development of safe immunotherapeutic medication. The objective of this study was to identify which pollen allergens are responsible for the T-cell activation in atopic patients with pollinosis and to determine whether the same allergens are responsible for the IgE-mediated reactions. Thirty-seven patients with pollinosis and thirteen non-allergic subjects were recruited. Peripheral blood samples were collected out of the pollen season. T-cell responses (IFN-γ production) towards different pollen allergens and levels of pollen specific IgE and IgG in the sera were measured by ELISA. The T-cell reactivity in most patients was directed towards the grass pollen B1, tree pollen I and autumn pollen B5. 70% of the allergic individuals responded to allergens from Lolium perenne, 84% to Dactylis glomerata, 11% to Phleum pratense, 65% to Betula pendula and 70% to Taxus baccata. Elevated serum levels of specific IgE in the allergic patients (p<0.01) were measured against the tree pollen I and autumn pollen, which include widespread deciduous trees (birch, willow, poplar and yew) and Artemisia absinthium, respectively. Our results show that the T-cell reactivity and antibody responses may be directed towards different or the same allergens. In addition, we propose that short synthetic peptides, which contain overlapping T- and B-cell epitopes can be used for specific immunotherapy treatment if they lack antibody recognition domains. | |
| dc.identifier.citation | Kostova Zh., Batsalova T., Moten D., Teneva I., Dzhambazov B. 2015. Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis. – Journal of BioScience and Biotechnology, 4(3): 271-283. | |
| dc.identifier.issn | 1314-6246 | |
| dc.identifier.uri | https://doi.uni-plovdiv.bg/handle/store/143 | |
| dc.language.iso | en | |
| dc.publisher | Plovdiv University Press “Paisii Hilendarski” | |
| dc.subject | pollen allergy | |
| dc.subject | T-cell responses | |
| dc.subject | B-cell responses | |
| dc.subject | allergens | |
| dc.title | Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis | |
| dc.type | Article |