Production, purification and characterization of thermostable α-amylase from soil isolate Bacillus sp. strain B-10

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dc.contributor.authorSingh, Ravindra Nath
dc.contributor.authorBahuguna, Ashutosh
dc.contributor.authorChauhan, Peeyush
dc.contributor.authorSharma, Vijay Kumar
dc.contributor.authorKaur, Satinder
dc.contributor.authorSingh, Santosh Kumar
dc.contributor.authorKhan, Amir
dc.date.accessioned2024-05-29T19:19:38Z
dc.date.available2024-05-29T19:19:38Z
dc.date.issued2016-01-03
dc.description.abstractA bacterial strain B-10 that produces α-amylase was isolated from compost and kitchen waste receiving agricultural soil. Based on microbiological and biochemical tests the isolate B-10 was identified as Bacillus sp. Alpha-amylase produced by this isolate was purified by (NH4)2SO4 precipitation and DEAE cellulose ion-exchange chromatography showing 15.91 and 48.21 fold purification, respectively. SDS-PAGE of the purified enzyme confirmed the purification and monomeric nature of the enzyme. The purified α-amylase showed maximum activity at pH 7 and temperature 50°C. The enzyme was significantly active in the temperature range of 30-60°C for the studied period of 2 h. During the incubation of purified enzyme at pH ranging from 5 to 10 for 24 h the maximum stability was observed at pH 7 followed by pH 8, whereas at extreme pH, the stability was very poor. Km and Vmax were found to be 1.4 mg/mL and 6.2 U/mL, respectively.
dc.identifier.issn1314-6246
dc.identifier.urihttps://doi.uni-plovdiv.bg/handle/store/158
dc.language.isoen
dc.publisherPlovdiv University Press “Paisii Hilendarski”
dc.subjectBacillus
dc.subjectα-amylase
dc.subjectSDS-PAGE
dc.subjection exchange chromatography
dc.subjectMichaelis constant
dc.subjectKm
dc.titleProduction, purification and characterization of thermostable α-amylase from soil isolate Bacillus sp. strain B-10
dc.typeArticle
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