A novel polymerase chain reaction (PCR) based assay for authentication of cell lines or tissues from human, pig and chicken origin

dc.contributor.authorGorenjak, Mario
dc.contributor.authorTrapecar, Martin
dc.contributor.authorGradisnik, Lidija
dc.contributor.authorSkok, Pavel
dc.contributor.authorCencic, Avrelija
dc.date.accessioned2024-05-22T16:07:32Z
dc.date.available2024-05-22T16:07:32Z
dc.date.issued2012-05-11
dc.description.abstractA polymerase chain reaction based assay was developed for authentication of cell lines or tissues from human, pig and chicken origin. Specificity was achieved by species specific primer design targeting the mitochondrial D-loop sequence. Amplicon sizes were 114 bp, 169 bp and 645-648 bp for chicken, human and pig derived cell lines, respectively. Primers were tested for species specificity and non-specificity between haplogroups of the same organisms using BLAST tool and subsequently for cross amplification DNA extracted from human, chicken and pig venous blood as a positive control. Primers were also amplifying specific products in DNA extracted from individual cell line in both functional cell models and intentionally mixed cell lines consisting functional cell models. The PCR assay developed in this study represents a low-cost species specific end-point PCR based assay of the mitochondrial D-loop for the authentication of the cell line origin.
dc.identifier.issn1314-6246
dc.identifier.urihttps://doi.uni-plovdiv.bg/handle/store/8
dc.language.isoen
dc.publisherPlovdiv University
Press "Paisii Hilendarski"
dc.subjectPolymerase chain reaction
dc.subjectmitochondrial D-loop
dc.subjectfunctional cell model
dc.subjectcell line authentication
dc.subjecthuman
dc.subjectpig
dc.subjectchicken
dc.titleA novel polymerase chain reaction (PCR) based assay for authentication of cell lines or tissues from human, pig and chicken origin
dc.typeArticle
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